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CrossLaps® for Culture (CTX-I) ELISA

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  • 产品名称:CrossLaps® for Culture (CTX-I) ELISA
  • 产品型号:AC-07F1
  • 产品展商:其它品牌
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简单介绍

品名:CrossLaps® for Culture (CTX-I) ELISA 货号:AC-07F1Code: AC-07F1 Certification: Research Use Only Clinical Area: Animal Research Type: Manual Format: ELISA RUO/IVD: RUO Number of Tests: 96 (40 samples in duplicate) Sample Type: Cell Culture Supernatant Sample Volume: 50 μL Pre-diluted (1:5) Assay Range: 0-112.7 nm

产品描述
Code: AC-07F1
Certification:

Research Use Only

Clinical Area: Animal Research
Type: Manual
Format: ELISA
RUO/IVD: RUO
Number of Tests: 96 (40 samples in duplicate)
Sample Type: Cell Culture Supernatant
Sample Volume: 50 μL Pre-diluted (1:5)
Assay Range: 0-112.7 nm

Unique Features

  • Screening of Anti-Resorptive Drugs1,2,3
  • High throughput screening of osteoclastic resorptive activity
  • Multiple testing of single osteoclast cultures over time
  • Objective assessment of in vitro bone resorption
  • High correlation to pit area
  • Dynamic monitoring of bone cultures
  • Improved lab productivity due to multi-well screening formats
  • Easy to perform – one step incubation
  • A complete assay panel supporting bone research

The test is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of degradation products of C-terminal telopeptides of type I collagen in bone cell culture supernatants.

Type I collagen accounts for more than 90% of the organic matrix of bone. During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation.

The resorption process can be studied in vitro by culturing bone cells on devitalised slices of bone or dentin.

The CrossLaps® for Culture (CTX-I) ELISA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption occur in the circulation as modified di-peptides. These modified (β-isomerised) and cross-linked di-peptides (Glu-Lys-Ala-His-Asp-β-Gly-Gly-Arg) must be covalently cross-linked through the lysine residue for signal in the CrossLaps® for Culture ELISA.

This epitope is present in type I collagen of many species, including human, bovine, elephant and chicken. However, it is not present in rat and mouse.

Zhuo Y et al., Inhibition of bone resorption by the cathepsin K inhibitor odanacatib is fully reversible. Bone. 2014 Jul 16;67C:269-280.

Leung P et al., The effects of the cathepsin K inhibitor odanacatib on osteoclastic bone resorption and vesicular trafficking. Bone. 2011 Oct;49(4):623-35.

Schaller S et al., In vitro, ex vivo, and in vivo methodological approaches for studying therapeutic targets of osteoporosis and degenerative joint diseases: how biomarkers can assist? Assay Drug Dev Technol. 2005 Oct;3(5):553-80.

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